Rituximab antibody (HRP)

Catalog No. ABIN5541687

The Rat Monoclonal anti-Rituximab antibody has been validated for EIA. It is suitable to detect Rituximab in samples from .

Quick Overview for Rituximab antibody (HRP) (ABIN5541687)

Target: Rituximab

Reactivity: Please inquire

Host: Rat

Clonality: Monoclonal

Conjugate: This Rituximab antibody is conjugated to HRP

Application: Enzyme Immunoassay (EIA)

Clone: MB2 A4

Product Details anti-Rituximab Antibody (HRP)

Specificity: This antibody specifically recognises the idiotypic determinants expressed by the Rituximab humanised monoclonal antibody. This antibody does not recognise other CD20 antibodies. It has been used to detect Rituximab bound to the surface of the Raji B cell line, however detection of Rituximab bound 'in vivo' to B-CLL cells has not been demonstrated. It is possible that complement deposition on Rituximab opsonised cells inhibits binding of the anti-Rituximab antibody to cell bound Rituximab (3). Clone MB2A4 has been used in ELISA assays to monitor the levels of rituximab in patient serum following therapy (4,5).

Purification: Affinity chromatography on Protein G

Immunogen: F(ab)2 fragment of Rituximab

Isotype: IgG2a

Application Details

Application Notes: ELISA: 1/20000-1/20000.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C

Storage Comment: Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Rituximab

Target: Rituximab

Target Type: Antibody

Background: Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen.